# Unable to get any data Wnt1 or Wnt10b in R-Studio
**URL:** https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311
**Category:** Help
**Tags:** R
**Created:** [July 21, 2022, 4:52am UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311 "2022-07-21T04:52:28Z")
**Posts on this page:** 11
**Page:** 1
### Author:  [@Mark\_Sandwich](https://help.c-moor.org/u/Mark_Sandwich)
#### Post date: [July 21, 2022, 4:52am UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311/1 "2022-07-21T04:52:29Z")
My R-Studio crashed and for some reason, I’m able to get most Wnt genes to plot their expression except for Wnt1 and Wnt10b, both of which are central to my poster. I brushed off the crash message thinking it was a regular one so I didn’t see what it said. I also created a brand new container but I still can’t get any expression out of the two genes and don’t get any plot results when I run stuff in the Console. Instead, I get graphs like the one below, which show no expression at all.
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### Author:  [@ShamounDivanyan](https://help.c-moor.org/u/ShamounDivanyan)
#### Post date: [July 21, 2022, 5:37am UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311/2 "2022-07-21T05:37:02Z")
Ive had this happen to me multiple times for a gene I was interested in, MSTN,
This just means that there is absolutely no expression (or at least no perceived expression) of your gene in the MIXED GASTRULATION dataset.
If you would like to load the FULL DATASET try this code:
load(“~/workspace/c\_moor\_data/gastrulation/gastrulation\_sce.rda”)
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### Author:  [@Mark\_Sandwich](https://help.c-moor.org/u/Mark_Sandwich)
#### Post date: [July 21, 2022, 5:52am UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311/3 "2022-07-21T05:52:35Z")
Do you know where I would include that line? I entered it here but I got an error.
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### Author:  [@LindsayHayes](https://help.c-moor.org/u/LindsayHayes)
#### Post date: [July 21, 2022, 12:45pm UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311/4 "2022-07-21T12:45:52Z")
It may be because this is only the mixed gastrulation sample instead of the full dataset. If you plot the full dataset with all 139331 cells you should see some signals.
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### Author:  [@LindsayHayes](https://help.c-moor.org/u/LindsayHayes)
#### Post date: [July 21, 2022, 12:49pm UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311/5 "2022-07-21T12:49:55Z")
[@CCC\_2022\_Summer](https://help.c-moor.org/groups/ccc_2022_summer)
**! Remember to delete any “dump” files or you will run out of space !**
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### Author:  [@Mark\_Sandwich](https://help.c-moor.org/u/Mark_Sandwich)
#### Post date: [July 21, 2022, 2:42pm UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311/6 "2022-07-21T14:42:48Z")
How do we switch the dataset we are working with again? Does it have to do with the
`sce <- readRSD` line?
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### Author:  [@LindsayHayes](https://help.c-moor.org/u/LindsayHayes)
#### Post date: [July 21, 2022, 2:59pm UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311/7 "2022-07-21T14:59:06Z")
I use this line to load the full dataset
```auto
load("~/workspace/c_moor_data/gastrulation/gastrulation_sce.rda")
```
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### Author:  [@Mark\_Sandwich](https://help.c-moor.org/u/Mark_Sandwich)
#### Post date: [July 21, 2022, 3:07pm UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311/8 "2022-07-21T15:07:42Z")
I added the line but for whatever reason, I don’t have that file in my C\_MOOR/c\_moor\_ccc\_su22/ folder. I only have the one that ends in “.RDS”
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### Author:  [@LindsayHayes](https://help.c-moor.org/u/LindsayHayes)
#### Post date: [July 21, 2022, 3:22pm UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311/9 "2022-07-21T15:22:12Z")
> [@LindsayHayes](#):
>
> `load("~/workspace/c_moor_data/gastrulation/gastrulation_sce.rda")`
Does this command work?
This file is not in the su22 folder. It is in the workspace/c\_moor\_data/gastrulation folder.
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### Author:  [@LindsayHayes](https://help.c-moor.org/u/LindsayHayes)
#### Post date: [July 21, 2022, 3:23pm UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311/10 "2022-07-21T15:23:13Z")
remember you will also need to run the `sce <- logNormCounts(sce)` command to log normalize the data before you can use it.
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### Author:  [@Mark\_Sandwich](https://help.c-moor.org/u/Mark_Sandwich)
#### Post date: [July 21, 2022, 3:34pm UTC](https://help.c-moor.org/t/unable-to-get-any-data-wnt1-or-wnt10b-in-r-studio/311/11 "2022-07-21T15:34:52Z")
Ah okay, that ended working, thank you very much!