- What I did: I wrote up the original paragraphs in each section, and these paragraphs were edited by Amber and Spoorthi. I prepared the abstract image, workflow image, and bar chart image along with their captions. I also made the figure captions for the other images. What I found most interesting was the representation of the abundances of the krona pie chart. We had to rerun our workflows a couple times to get the abundances, and it was interesting to see how vast of a difference the bacillota abundances were between the different samples.
- Some other things that could be continued in the project is picking datasets associated with other conditions, developing other visual representations like heat map, looking at the abundances of other phylums than just bacillota and more. I would look for long-read datasets associated with other conditions and look to another phylum’s abundance.
What I did: I helped edit the paragraphs throughout the poster. I also made the metadata table for our datasets, where we organized the sample descriptions, SRA sequence IDs, sequencing tools, and number of bases. I also worked on the Galaxy analysis part of the project. I ran the workflows on our datasets to generate the Krona pie charts. I then connected the Galaxy results back to our hypothesis by comparing the levels of Bacillota and related groups across the samples.
How you can help: Others could expand on this project by using a larger number of samples for each group instead of only comparing one dataset per condition. This would make the results more reliable because there would be more data to work with. I would also make sure the datasets are more consistent in the type of sequencing, sample type, and similar processing steps.